全文获取类型
收费全文 | 1018篇 |
免费 | 52篇 |
国内免费 | 1篇 |
出版年
2022年 | 7篇 |
2021年 | 7篇 |
2020年 | 6篇 |
2019年 | 16篇 |
2018年 | 38篇 |
2017年 | 35篇 |
2016年 | 29篇 |
2015年 | 30篇 |
2014年 | 53篇 |
2013年 | 78篇 |
2012年 | 68篇 |
2011年 | 82篇 |
2010年 | 58篇 |
2009年 | 48篇 |
2008年 | 49篇 |
2007年 | 41篇 |
2006年 | 46篇 |
2005年 | 40篇 |
2004年 | 54篇 |
2003年 | 54篇 |
2002年 | 57篇 |
2001年 | 45篇 |
2000年 | 27篇 |
1999年 | 22篇 |
1998年 | 5篇 |
1996年 | 2篇 |
1992年 | 9篇 |
1991年 | 9篇 |
1990年 | 16篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 3篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有1071条查询结果,搜索用时 15 毫秒
11.
Magdalena Brzezowska Francesca Bernardi Nicola Gaggelli Gianni Valensin 《Journal of inorganic biochemistry》2010,104(2):193-198
Teicoplanin, a member of the “last chance” antibiotic family has a similar structure and the same mechanism of action as parent drug vancomycin, which is proved to be an effective binder of Cu(II) ions. However, the potentiometric and spectroscopic studies (UV-visible, CD, NMR) have shown that the modification of the N-terminal structure of the peptide backbone in teicoplanin affects considerably the binding ability towards Cu(II) ions. While vancomycin forms almost instantly the stable 3 N complex species involving the N-terminal and two amide nitrogen donors, in case of teicoplanin only two nitrogen donors derived from the N-terminal amino group and adjacent peptide bond are coordinated to Cu(II) ion within the whole pH range studied. The major factor influencing the binding mode is most likely the structure of the N-terminus of the peptide unit in the antibiotic ligand. 相似文献
12.
Martyna Kielmas Zbigniew SzewczukPiotr Stefanowicz 《Biochemical and biophysical research communications》2013
Although in vivo glycation proceeds in complex mixture of proteins, previous studies did not take in consideration the influence of protein–protein interaction on Maillard reaction. The aim of our study was to test the influence of human serum albumin (HSA) on glycation of fibrinogen. The isotopic labeling using [13C6] glucose combined with LC-MS were applied as tool for identification possible glycation sites in fibrinogen and for evaluation the effect of HSA on the glycation level of selected amino acids in fibrinogen. 相似文献
13.
J. Orpiszewski C. Hebda J. Szykua R. Powls S. Clasper H.H. Rees 《FEMS microbiology letters》1991,82(2):233-236
Six out of seven tested strains of mycobacteria transformed abietic acid to methyl abietate in shake culture. The conversion carried out by Mycobacterium sp. MB 3683 was induced by the substrate and stimulated by methionine. Fractionation of the cell extract of Mycobacterium sp. MB 3683 on DEAE cellulose, Ultrogel AcA 44 and MONO Q resulted in the separation of three distinct methyltransferase activities which could also esterify palmitic acid. The separated forms of the methyltransferase exhibited different activities towards these two substrates. 相似文献
14.
Acinetobacter baumannii causes serious infections especially in immunocompromised and/or hospitalized patients. Several A. baumannii strains are multidrug resistant and infect wounds, bones, and the respiratory tract. Current studies are focused on finding new effective agents against A. baumannii. Phage therapy is a promising means to fight this bacterium and many studies on procuring and applying new phages against A. baumannii are currently being conducted. As shown in animal models, phages against multidrug-resistant A. baumannii may control bacterial infections caused by this pathogen and may be a real hope to solve this dangerous health problem. 相似文献
15.
Aikaterini Pagoni Theohari Daliani Katarzyna Macegoniuk Stamatia Vassiliou Łukasz Berlicki 《Bioorganic & medicinal chemistry letters》2019,29(9):1085-1089
Targeted covalent inhibitors of urease were developed on the basis of the catechol structure. Forty amide and ester derivatives of 3,4-dihydroxyphenylacetic acid, caffeic acid, ferulic acid and gallic acid were obtained and screened against Sporosarcinia pasteurii urease. The most active compound, namely propargyl ester of 3,4-dihydroxyphenylacetic acid exhibited IC50?=?518?nM and?=?1379?M?1?s?1. Inhibitory activity of this compound was better and toxicity lower than those obtained for the starting compound – catechol. The molecular modelling studies revealed a mode of binding consistent with structure-activity relationships. 相似文献
16.
N‐terminal guanidinylation of the cyclic 1,4‐ureido‐deltorphin analogues: the synthesis,receptor binding studies,and resistance to proteolytic digestion 下载免费PDF全文
Krzysztof Bańkowski Olga M. Michalak Anna Leśniak Katarzyna E. Filip Piotr Cmoch Zbigniew Szewczuk Piotr Stefanowicz Jan Izdebski 《Journal of peptide science》2015,21(6):467-475
The synthesis of a series of N‐guanidinylated cyclic ureidopeptides, analogues of 1,4‐ureido‐deltorphin/dermorphine tetrapeptide is described. The δ‐ and μ‐opioid receptor affinity of new guanidinylated analogues and their non‐guanidinylated precursors was determined by the displacement radioligand binding experiments. Our results indicate that the guanidinylation of cyclic 1,4‐ureidodeltorphin peptide analogues does not exhibit a uniform influence on the opioid receptor binding properties, similarly as reported earlier for some linear peptides. All analogues were also tested for their in vitro resistance to proteolysis during incubation with large excess of chymotrypsin, pepsin, and papain by means of mass spectroscopy. Guanidinylated ureidopeptides 1G–4G showed mixed μ agonist/δ agonist properties and high enzymatic stability indicating their potential as therapeutic agents for treatment of pain. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
17.
Plant invasion is a major threat to the integrity of an ecosystem. Exceptionally successful invaders in Europe are the American species of Solidago genus. In this study we examined growth, reproduction, and phenology of Solidago species, of American origin, growing in central Europe (S. altissima, S. canadensis, S. gigantea, S. graminifolia). These taxa were compared with two native species: Solidago virgaurea and Tanacetum vulgare. We observed high differentiation in height, number of shoots, and biomass production between individuals within taxa. Generally, the invasive species produced substantially (two to five times) more biomass than the native ones, being statistically significant in the comparison of alien Solidago graminifolia and S. gigantea versus native S. virgaurea and T. vulgare. The ratio of biomass of reproductive parts to overall biomass varied considerably among years, but generally the lowest one was for Solidago altissima, and the highest for S. graminifolia. It shows a lack of a clear pattern of differentiation between alien and native species in terms of biomass investment in reproduction. We observed a general tendency of allocation of a major part of biomass in rhizomes by phalanx species (S. graminifolia and S. gigantea), while species with guerrilla strategy (S. altissima and S. canadensis) invested more biomass in stems and leaves. However, because of the high variability there was no clear, stable pattern of statistically significant differences between these two groups. The results suggest that S. graminifolia reveals a strong potential of invasion, in spite of its, so far limited, distribution in Europe. 相似文献
18.
Magdalena Wysocka Anna Wojtysiak Małgorzata Okońska Natalia Gruba Mirosław Jarząb Tomasz Wenta Barbara Lipińska Reneta Grzywa Marcin Sieńczyk Krzysztof Rolka Adam Lesner 《Analytical biochemistry》2015
HtrA2 belongs to the HtrA (high temperature requirement A) family of ATP-independent serine proteases. The primary function of HtrA2 includes maintaining the mitochondria homeostasis, cell death (by apoptosis, necrosis, or anoikis), and contribution to the cell signaling. Several recent reports have shown involvement of HtrA2 in development of cancer and neurodegenerative disorders. Here, we describe the profiling of HtrA2 protease substrate specificity via the combinatorial chemistry approach that led to the selection of novel intramolecularly quenched substrates. For all synthesized compounds, the highest HtrA2-mediated hydrolysis efficiency and selectivity among tested HtrA family members was observed for ABZ-Ile-Met-Thr-Abu-Tyr-Met-Phe-Tyr(3-NO2)-NH2, which displayed a specificity constant kcat/KM value of 14,535 M−1 s−1. 相似文献
19.
20.